Journal: Communications Biology

Article Title: Distinct neuropeptide-receptor modules regulate a sex-specific behavioral response to a pheromone

doi: 10.1038/s42003-021-02547-7

Figure Lengend Snippet: a Design of the Single Worm Attraction Assay (SWAA). The outer 40 wells of a 48-well suspension cell culture plate are seeded with NGM agar and a thin lawn of OP50 E. coli . A random block design results in spatial control (light gray), vehicle control (dark gray), and ascaroside (purple) containing wells. Quadrants are recorded for 15 m. (Inset) The structure of ascr#8. b Raw dwell times of males of N2 (blue), him-5 (red), him-8 (purple), osm-3;him-5 (orange) in SWAA. c Transformed log(fold-change) of male dwell time data. d Raw dwell time of hermaphrodites across strains and e log(fold-change) of SWAA data of hermaphrodites. Light gray denotes spatial controls (“S”) (when applicable), dark grey denotes vehicle controls (“V”), colors denote ascr#8 values (“A”). ♂ denotes male data, ⚥ denotes hermaphrodite data. For all figures: Error bars denote SEM. n ≥ 5. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, unless denoted otherwise. For 1b : ++++ p < 0.0001, vehicle vs. spatial control.

Article Snippet: The outer forty wells of a 48-well suspension culture plate (Olympus Plastics, Cat #: 25-103) were seeded with 200 µL of standard NGM agar.

Techniques: Cell Culture, Blocking Assay, Transformation Assay